Advances in New Technology for Targeted Modification of by Feng Zhang, Holger Puchta, James G. Thomson

By Feng Zhang, Holger Puchta, James G. Thomson

During the last 50 years, biotechnology has been the foremost driver for expanding crop productivity. Particularly, advances in plant genetic engineering applied sciences have spread out mammoth new possibilities for plant researchers and breeders to create new crop types with fascinating traits. Recent improvement of exact genome amendment tools, comparable to certain gene knock-out/knock-in and certain gene alternative, strikes genetic engineering to a different point and provides much more potentials for bettering crop production. The paintings offers an summary of the most recent advances on targeted genomic engineering applied sciences in crops. subject matters comprise recombinase and engineered nucleases-mediated specified amendment, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. eventually, demanding situations and affects of the recent applied sciences on current rules for genetic amendment organisms (GMOs) could be mentioned.

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Mol Ther 19(4):694–702 Heath PJ, Stephens KM, Monnat RJ Jr, Stoddard BL (1997) The structure of I-Crel, a group I intron-encoded homing endonuclease. Nat Struct Biol 4(6):468–476 2 Engineering Meganuclease for Precise Plant Genome Modification 37 Hu D, Crist M, Duan X, Gimble FS (1999) Mapping of a DNA binding region of the PI-SceI homing endonuclease by affinity cleavage and alanine-scanning mutagenesis. Biochemistry 38(39):12621–12628 Ichiyanagi K, Ishino Y, Ariyoshi M, Komori K, Morikawa K (2000) Crystal structure of an archaeal intein-encoded homing endonuclease PI-PfuI.

2010; Tzfira et al. 2012). ZFNs functions as dimers while each monomer contains a zinc finger DNA-binding domain and a nonspecific FokI nuclease domain (Fig. 1) (Kim et al. 1996; Bibikova et al. 2001). The zinc finger domain typically consists of three to four zinc finger DNAbinding motifs, with each one recognizing 3-nt DNA sequence. Thus, a ZFN pair can recognize an 18 or 24 bp DNA sequence, which is highly specific in the genome. The modularity of DNA binding by zinc fingers makes engineering site-specific ZFNs possible.

Mol Cell 2(4):469–476 Kleinstiver BP, Wang L, Wolfs JM et al (2014) The I-TevI nuclease and linker domains contribute to the specificity of monomeric TALENs. G3 (Bethesda) 4(6):1155–1165 Lyznik LA, Djukanovic V, Yang M, Jones S (2012) Double-strand break-induced targeted mutagenesis in plants. Methods Mol Biol 847:399–416 Menoret S, Fontaniere S, Jantz D et al (2013) Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases. FASEB J 27(2):703–711 Michael TP, Jackson S (2013) The first 50 plant genomes.

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