By Feng Zhang, Holger Puchta, James G. Thomson
During the last 50 years, biotechnology has been the foremost driver for expanding crop productivity. Particularly, advances in plant genetic engineering applied sciences have spread out mammoth new possibilities for plant researchers and breeders to create new crop types with fascinating traits. Recent improvement of exact genome amendment tools, comparable to certain gene knock-out/knock-in and certain gene alternative, strikes genetic engineering to a different point and provides much more potentials for bettering crop production. The paintings offers an summary of the most recent advances on targeted genomic engineering applied sciences in crops. subject matters comprise recombinase and engineered nucleases-mediated specified amendment, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. eventually, demanding situations and affects of the recent applied sciences on current rules for genetic amendment organisms (GMOs) could be mentioned.
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Additional info for Advances in New Technology for Targeted Modification of Plant Genomes
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2010; Tzfira et al. 2012). ZFNs functions as dimers while each monomer contains a zinc finger DNA-binding domain and a nonspecific FokI nuclease domain (Fig. 1) (Kim et al. 1996; Bibikova et al. 2001). The zinc finger domain typically consists of three to four zinc finger DNAbinding motifs, with each one recognizing 3-nt DNA sequence. Thus, a ZFN pair can recognize an 18 or 24 bp DNA sequence, which is highly specific in the genome. The modularity of DNA binding by zinc fingers makes engineering site-specific ZFNs possible.
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