Agrobacterium Protocols by Kevan M. A. Gartland, Michael R. Davey

By Kevan M. A. Gartland, Michael R. Davey

Agrobacterium Protocols bargains starting and skilled researchers the main complete choice of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the themes variety from the upkeep of bacterial tradition collections to points of the metabolism and body structure of reworked tissues and transgenic vegetation. Drawing at the paintings of major scientists from laboratories all over the world, Agrobacterium Protocols presents a wealth of thoughts for introducing particular DNA sequences into goal plant species and discusses the environmental implications of genetically engineered crops. Its specified systems will facilitate swift move of complex recommendations to different laboratories and their exploitation in primary and utilized plant biology.

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References 1 Ream, L. W. and Gordon, M P. (1982) Crown gall drsease and prospects for genetic mampulatron of plants. Science 218, 854-859. 2 Schell, J (1987) Transgenic plants as a tools to study the molecular organization of plant genes. Science 237, 1176-l 183 3 Hooykaas, P. J. J and Schilperoort, R. A. (1992) Agrobacterium and plant genetic engineering. Plant Mol. Biol. 19,15-38. , and Schell, J. (1983) Ti plasmrd vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacrty.

Since the CAT assay is very sensitive and most plants do not have endogenous CAT activity, this reporter system has been widely used for studying various promoter elements. However, some plant species contain an inhibitor for An 56 the CAT enzyme activity that may be inactivated by brief heating of the plant extract at 55-65°C. A similar set of binary vectors for the GUS reporter gene have also been widely used for promoter analysis (20). In both systems, addition of an extra peptide sequence at the amino terminal ends does not destroy the enzyme activities.

1983) Ti plasmrd vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacrty. EMBO J 2,2143-2150. 5. , Murphy, E. B , Boberts, J L , Gustafson, G. , and Malcolm, S. K (1985) Resistance to hygromycin B A new marker for plant transformation studres. Plant Mol Biol 5, 103-108. 6 Holt, J. , Powles, S. , and Holtum, A M. (1993) Mechanisms and agronomic aspect of herbicide resistance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 44,203-229. , Hirsch, P.

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